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cell lines hmgu1 induced pluripotent stem cells cf ipsc hmgu isfi001 a n2a cells atcc ccl 131 hmgu1 induced pluripotent stem cells map1b wt  (ATCC)


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    ATCC cell lines hmgu1 induced pluripotent stem cells cf ipsc hmgu isfi001 a n2a cells atcc ccl 131 hmgu1 induced pluripotent stem cells map1b wt
    Cell Lines Hmgu1 Induced Pluripotent Stem Cells Cf Ipsc Hmgu Isfi001 A N2a Cells Atcc Ccl 131 Hmgu1 Induced Pluripotent Stem Cells Map1b Wt, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hipsc+line/Neuro-2a/pm42276043-789-208-220
    Average 99 stars, based on 4226 article reviews
    cell lines hmgu1 induced pluripotent stem cells cf ipsc hmgu isfi001 a n2a cells atcc ccl 131 hmgu1 induced pluripotent stem cells map1b wt - by Bioz Stars, 2026-09
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    Article Title: Engineering a Wnt-activated osteomimetic microenvironment with iPSC-derived MSCs for synergistic bone regeneration, angiogenesis, and neurogenesis.
    Article Snippet: To advance the bioactivity of artificial bone grafts, human induced pluripotent stem cell (hiPSC)-derived mesenchymal stem cells (iMSCs) are ideal seed cells.. However, the instability in iMSC generation and their limited osteogenic capacity challenge their clinical application.. In this study, we applied a novel strategy that combines a stable iMSC induction method that mimics the developmental process with an osteocytic Wnt-based osteogenic microenvironment, with the aim of creating a scalable, mechanistically defined platform to enhance bone regenerative efficiency.

    Article Title: Establishing stable and highly osteogenic hiPSC-derived MSCs for 3D-printed bone graft through microenvironment modulation by CHIR99021-treated osteocytes
    Article Snippet: .. The hiPSC line (DYR0100, derived from the foreskin fibroblast cell line SCRC-1041) was purchased from ATCC (Manassas, VA, USA). ..

    Article Title: The secreted protein Amuc_1409 from Akkermansia muciniphila improves gut health through intestinal stem cell regulation
    Article Snippet: For Cdh1 deletion in Lgr5 + stem cells, the organoids generated from Lgr5-CreERT2;Cdh1 fl/fl mice were treated with 4-hydroxytamoxifen (4-OHT, 1 μM, Selleck Chemicals, Houston, TX, USA) for 6 consecutive days. .. This research received approval from the Korean Public Institutional Review Board (IRB numbers: P01-201409-ES-01-09, P01-201609-31-002). hIOs were generated through the differentiation of the H9 (WA09, XX; WiCell Research Institute, Madison, WI) or H1 (WA01, XY; WiCell Research Institute) hESC line and a hiPSC line derived from CRL-2097 (CCD-1079SK, skin fibroblast; ATCC, Rockville, MD, USA), following established protocols , . hPSC-derived hIOs were generated as described previously . .. Briefly, the differentiation of hPSCs into definitive endoderms (DEs) was induced by treatment with 100 ng/mL activin A (R&D Systems, Minneapolis, MN, USA) for 3 days in RPMI1640 (Thermo Fisher Scientific) containing 0, 0.2, and 2% (v/v) fetal bovine serum (FBS, Thermo Fisher Scientific).

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    Article Title: The secreted protein Amuc_1409 from Akkermansia muciniphila improves gut health through intestinal stem cell regulation
    Article Snippet: For Cdh1 deletion in Lgr5 + stem cells, the organoids generated from Lgr5-CreERT2;Cdh1 fl/fl mice were treated with 4-hydroxytamoxifen (4-OHT, 1 μM, Selleck Chemicals, Houston, TX, USA) for 6 consecutive days. .. This research received approval from the Korean Public Institutional Review Board (IRB numbers: P01-201409-ES-01-09, P01-201609-31-002). hIOs were generated through the differentiation of the H9 (WA09, XX; WiCell Research Institute, Madison, WI) or H1 (WA01, XY; WiCell Research Institute) hESC line and a hiPSC line derived from CRL-2097 (CCD-1079SK, skin fibroblast; ATCC, Rockville, MD, USA), following established protocols , . hPSC-derived hIOs were generated as described previously . .. Briefly, the differentiation of hPSCs into definitive endoderms (DEs) was induced by treatment with 100 ng/mL activin A (R&D Systems, Minneapolis, MN, USA) for 3 days in RPMI1640 (Thermo Fisher Scientific) containing 0, 0.2, and 2% (v/v) fetal bovine serum (FBS, Thermo Fisher Scientific).

    Article Title: Using CRISPR/Cas9 to generate a heterozygous COL2A1 p.R719C iPSC line (MCRIi019-A-6) model of human precocious osteoarthritis.
    Article Snippet: .. This hiPSC line was generated from ATCC®CRL-1502TM fibroblasts by the MCRI Gene Editing Core Facility, which is supported by the Stafford Fox Medical Research Foundation. ..

    Article Title: Helicobacter pylori VacA-induced mitochondrial damage in the gastric pit cells of the antrum and therapeutic rescue.
    Article Snippet: The hESC line H9 (WA09) was purchased from WiCell and its use approved by the Public Institutional Review Board designated by the Ministry of Health and Welfare (P01-201409-ES-01). .. The hiPSC line was generated by reprogramming human fibroblasts (CRL-2097, American Type Culture Collection), using episomal vectors, and characterized as previously described [17]. ..



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    A) Time-lapse images of a live plated neurocrestoid with nuclei stained with SPY650-DNA. B) Representative fluorescence images at day 14 of plated neurocrestoids immunostained for SOX10 and nuclei (DAPI). C) Bar chart depicting percentage of SOX10 positive cells amongst the total nuclei (DAPI) in plated neurocrestoids at day 14 across experiments from 3 independent hPSC lines (mean ± S.D). Each point represents the average across n=5-18 organoids per individual experiment. F) Neurocrestoids plated at day 14 and stained against HOXC9, SOX10 and nuclei (DAPI). E) Representative immunofluorescence images of mouse trunk explant and human neurocrestoid-derived NCCs stained for SOX10. F) Bar charts indicating the area, circularity and solidity values for neurocrestoid-derived human and mouse trunk NC nuclei (mean ± S.D). Points represent the average value per field of view. For the human datasets, a total of 38 datapoints are plotted representing the average value per field of view, 5 images per sample, 3-5 organoids per cell line from 3 <t>hiPSC</t> <t>lines</t> (Control 3, KOLFC21 and KOLF2.1J) in 3 experimental blocks. The mouse dataset consists of the average of 10 cells per image, 10 images per explant in 3 mouse trunk NC explants cultured for 48 to 72 hours in 2 experimental blocks. Non-parametric Mann-Whitney test to test for significance between conditions, **P<0.01, ns: non-significant.
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    A) Time-lapse images of a live plated neurocrestoid with nuclei stained with SPY650-DNA. B) Representative fluorescence images at day 14 of plated neurocrestoids immunostained for SOX10 and nuclei (DAPI). C) Bar chart depicting percentage of SOX10 positive cells amongst the total nuclei (DAPI) in plated neurocrestoids at day 14 across experiments from 3 independent hPSC lines (mean ± S.D). Each point represents the average across n=5-18 organoids per individual experiment. F) Neurocrestoids plated at day 14 and stained against HOXC9, SOX10 and nuclei (DAPI). E) Representative immunofluorescence images of mouse trunk explant and human neurocrestoid-derived NCCs stained for SOX10. F) Bar charts indicating the area, circularity and solidity values for neurocrestoid-derived human and mouse trunk NC nuclei (mean ± S.D). Points represent the average value per field of view. For the human datasets, a total of 38 datapoints are plotted representing the average value per field of view, 5 images per sample, 3-5 organoids per cell line from 3 hiPSC lines (Control 3, KOLFC21 and KOLF2.1J) in 3 experimental blocks. The mouse dataset consists of the average of 10 cells per image, 10 images per explant in 3 mouse trunk NC explants cultured for 48 to 72 hours in 2 experimental blocks. Non-parametric Mann-Whitney test to test for significance between conditions, **P<0.01, ns: non-significant.

    Journal: bioRxiv

    Article Title: Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system

    doi: 10.64898/2026.05.04.721958

    Figure Lengend Snippet: A) Time-lapse images of a live plated neurocrestoid with nuclei stained with SPY650-DNA. B) Representative fluorescence images at day 14 of plated neurocrestoids immunostained for SOX10 and nuclei (DAPI). C) Bar chart depicting percentage of SOX10 positive cells amongst the total nuclei (DAPI) in plated neurocrestoids at day 14 across experiments from 3 independent hPSC lines (mean ± S.D). Each point represents the average across n=5-18 organoids per individual experiment. F) Neurocrestoids plated at day 14 and stained against HOXC9, SOX10 and nuclei (DAPI). E) Representative immunofluorescence images of mouse trunk explant and human neurocrestoid-derived NCCs stained for SOX10. F) Bar charts indicating the area, circularity and solidity values for neurocrestoid-derived human and mouse trunk NC nuclei (mean ± S.D). Points represent the average value per field of view. For the human datasets, a total of 38 datapoints are plotted representing the average value per field of view, 5 images per sample, 3-5 organoids per cell line from 3 hiPSC lines (Control 3, KOLFC21 and KOLF2.1J) in 3 experimental blocks. The mouse dataset consists of the average of 10 cells per image, 10 images per explant in 3 mouse trunk NC explants cultured for 48 to 72 hours in 2 experimental blocks. Non-parametric Mann-Whitney test to test for significance between conditions, **P<0.01, ns: non-significant.

    Article Snippet: HiPSC lines were obtained either from commercially available sources: Control 3 (ThermoFisher Scientific, A18945), KOLF2.1J (Jackson Laboratory, JIPSC1000), or kindly donated: KOLF2C1 (Maximiliano Gutierrez, The Francis Crick Institute).

    Techniques: Staining, Fluorescence, Immunofluorescence, Derivative Assay, Control, Cell Culture, MANN-WHITNEY